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KMID : 0545120000100040518
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 4 p.518 ~ p.521
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Metabolism of Dimethylphthalate by Aspergillus niger
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SHARANAGOUDA PRADEEPKUMAR
T. B. KAREGOUDAR
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Abstract
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Aspergillus niger is capable of metabolizing dimethylphthalate. The maximum weight of mycelium was observed after about 6-8 days of incubation. A TLC analysis revealed the accumulation of metabolites in the resting cell culture. Monomethylphthalate, phthalate, and protocatechuate were shown to be the intermediates by thin layer chromatographic and spectrophotometric analyses. The fungus metabolized dimethylphthalate through monomethylphthalate, phthalate, and protocatechuate as evidenced by the oxygen uptake and an enzymatic analysis. The terminal aromatic metabolite, protocatechuate, is metabolized via the ortho-cleavage pathway.
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